日粮能量和蛋白水平对滩羊脂肪组织PPARγ和FABP4 mRNA表达的影响

doi:10.11843/j.issn.0366-6964.2016.11.014

Abstract

Abstract:

The objective of this study was to research the effects of diet energy and protein levels on the mRNA expression of peroxisome proliferator-activated receptor γ(PPARγ) and fatty acid binding protein 4(FABP4) in the adipose tissues of Tan sheep. A total of 112(56♂,56♀) healthy Tan sheep with similar body weight were selected and allocated randomly to 4 groups(4 replicates in each group, and 7 sheep in each replicate). 3 stages(22-28, 29-35, 36-40 kg) was classified, and standard diets for each stage were designed according to body weight and expected daily gain in sheep feeding standard of NY/T(816-2004), 4 diets with energy and protein levels of 0.84×Standard(group I), 0.96×Standard(group II), 1.08×Standard(group III), 1.20×Standard(group IV) were assigned to feed sheep in each group, respectively. At the end of each stage, one sheep from each replicate was slaughtered, and samples of tail fat, perirenal fat and subcutaneous fat were collected for analyzing the mRNA expression of PPARγ and FABP4 by Real-time quantitative PCR. The results showed as follows: In tail fat, PPARγ and FABP4 mRNA expression levels was significantly higher in group II than other groups at the end of stage 22-28 and 36-40 kg(P<0.05); FABP4 mRNA expression levels was highest in group II, and lowest in group IV at the end of stage 29-35 kg(P<0.05). In perirenal fat, PPARγ mRNA expression levels was significnatly higher in group II, and significantly lower in group IV compared with group III at the end of 3 stages(P<0.05). FABP4 mRNA expression levels was higher in group II and III at the end of stage 22-28 kg(P<0.05); FABP4 mRNA expression levels was higher in group II compared with group IV at the end of stage 29-35 kg(P<0.05); FABP4 mRNA expression levels was higher in group I and II at the end of stage 36-40 kg(P<0.05). In subcutaneous fat, there was no significant difference between different groups at the end of 3 stages(P>0.05). These results indicated that the effect of diet energy and protein levels on PPARγ and FABP4 mRNA expression was greater in deep fat tissue(perirenal fat) than that in superficial fat tissue(subcutaneous fat) in Tan sheep. The diets with different energy and protein levels had an effect on PPARγ and FABP4 mRNA expression of tail fat in Tan sheep.

CLC Number:

S826
S816.4

WANG Xiao-fang, ZENG Jie, TIAN Chong-qi, WANG Xiao-long, YANG Yu-xin, CHEN Yu-lin，ZHANG En-ping. Effects of Diet Energy and Protein Levels on the mRNA Expression of PPARγ and FABP4 in Adipose Tissues of Tan Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(11): 2266-2273.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/10.11843/j.issn.0366-6964.2016.11.014

https://www.xmsyxb.com/EN/Y2016/V47/I11/2266

References

［1］GARIN-SHKOLNIK T, RUDICH A, HOTAMISLIGIL G S, et al. FABP4 attenuates PPARgamma and adipogenesis and is inversely correlated with PPARgamma in adipose tissues［J］. Diabetes, 2014, 63(3): 900-911.
［2］BAKHTIARIZADEH M R, MORADI-SHAHRBABAK M, EBRAHIMIE E. Underlying functional genomics of fat deposition in adipose tissue［J］. Gene, 2013, 521(1): 122-128.
［3］XU X, WEI X, YANG Y, et al. PPAR gamma, FAS, HSL mRNA and protein expression during Tan sheep fat-tail development［J］. Electron J Biotechnol, 2015, 18(2): 122-127.
［4］UYSAL K T, SCHEJA L, WIESBROCK S M, et al. Improved glucose and lipid metabolism in genetically obese mice lacking aP2［J］. Endocrinology, 2000, 141(9): 3388-3396.
［5］JONES J R, BARRICK C, KIM K A, et al. Deletion of PPARgamma in adipose tissues of mice protects against high fat diet-induced obesity and insulin resistance［J］. Proc Natl Acad Sci U S A, 2005, 102(17): 6207-6212.
［6］TONTONOZ P, SPIEGELMAN B M. Fat and beyond: The diverse biology of PPAR gamma［J］. Annu Rev Biochem, 2008, 77: 289-312.
［7］MORADI M H, NEJATI-JAVAREMI A, MORADI-SHAHRBABAK M, et al. Genomic scan of selective sweeps in thin and fat tail sheep breeds for identifying of candidate regions associated with fat deposition［J］. BMC Genet, 2012, 13: 10.
［8］ABDULLAH A Y, MUSALLAM H. Effect of different levels of energy on carcass composition and meat quality of male black goats kids［J］. Livest Sci, 2007, 107(1): 70-80.
［9］LEWIS R M, EMMANS G C, SIMM G. Effects of index selection on the performance and carcass composition of sheep given foods of different protein concentrations ad libitum［J］. Anim Sci, 2004, 78(2): 203-212.
［10］TAHMOORESPUR M, GHAZANFARI S, NOBARI K. Evaluation of adiponectin gene expression in the abdominal adipose tissue of broiler chickens: Feed restriction, dietary energy, and protein influences adiponectin messenger ribonucleic acid expression［J］. Poult Sci, 2010, 89(10): 2092-2100.
［11］PENG Q H, WANG Z S, TAN C, et al. Effects of different pomace and pulp dietary energy density on growth performance and intramuscular fat deposition relating mRNA expression in beef cattle［J］. J Food Agric Environ, 2012, 10(1): 404-407.
［12］ZHANG H B, ZHANG X F, WANG Z S, et al. Effects of dietary energy level on lipid metabolism-related gene expression in subcutaneous adipose tissue of Yellow breed × Simmental cattle［J］. Anim Sci J, 2015, 86(4): 392-400.
［13］XU X, WEI X, YANG Y, et al. mRNA transcription and protein expression of PPAR, FAS, and HSL in different parts of the carcass between fat-tailed and thin-tailed sheep［J］. Electron J Biotechnol, 2015, 18(3): 215-220.
［14］ZHAO S, WANG J, SONG X, et al. Impact of dietary protein on lipid metabolism-related gene expression in porcine adipose tissue［J］. Nutr Metab (Lond), 2010, 7: 6.
［15］许瑞霞, 高磊, 赵伟利, 等. FABP4基因在阿勒泰羊尾脂沉积与代谢模型中的表达变化规律［J］. 遗传, 2015,37(2): 174-182.
XU R X, GAO L, ZHAO W L, et al. Analysis of FABP4 expression pattern in rump fat deposition and metabolism of Altay sheep［J］. Hereditas (Beijing), 2015,37(2): 174-182.（in Chinese）
［16］LOPES P A, COSTA A S, COSTA P, et al. Contrasting cellularity on fat deposition in the subcutaneous adipose tissue and longissimus lumborum muscle from lean and fat pigs under dietary protein reduction［J］. Animal, 2014, 8(4): 629-637.
［17］张小丽. 猪背部浅层和背部深层脂肪组织全基因组甲基化研究［D］. 雅安：四川农业大学, 2013.
ZHANG X L. Genome-wide DNA methylation changes between the superficial and deep backfat tissues of the pig［D］. Ya’an：Sichuan Agricultural University, 2013. （in Chinese）
［18］吕刚. 不同饲喂方式下肉鸭体脂沉积规律及机制研究［D］. 雅安：四川农业大学, 2011.
LV G. The fat deposition of meat-type duck under different feeding forms and its mechanism［D］. Ya’an：Sichuan Agricultural University, 2011. （in Chinese）

[1]	WANG Haibo, ZHAN Jinshun, GU Zhiyong, CHEN Xinfeng, PAN Yue, JIA Haobin, ZHONG Xiaojun, LI Kairong, ZHAO Shengguo, HUO Junhong. Comparative Study on Meat Quality Characteristics of Three-Way Hybrid Sheep Charolais×Duper×Hu and Charolais×Australian White×Hu and Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 110-119.
[2]	DUAN Xiangru, KANG Jia, YANG Ruochen, SHAN Xinyu, LI Taichun, ZHAO Wen, ZHANG Yingjie, LIU Yueqin. Effect of L-cysteine on Proliferation, Apoptosis and the Secretion of Steroid Hormone in Ovine Ovarian Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 179-191.
[3]	SONG Pengyan, WANG Siwei, YUE Qiaoxian, ZHANG Yinliang, CHEN Xiaoyong, ZHOU Rongyan. Identification of oar-miR-200b Promoter and Effects on Mitochondrial Function in Follicular Granulosa Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5066-5076.
[4]	HAN Haoyuan, LI Shikai, YANG Ruiqiao, LI Manman, LI Jun, HA Si, ZHAO Jinyan, WEI Hongfang, QUAN Kai. Mining Key Candidate Genes for High Reproduction Performance of Huai Goats Based on Transcriptome Sequencing [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5077-5090.
[5]	CHEN Conghui, DUAN Chunhui, YANG Xinyu, XIA Cui, GUO Yunxia, JI Shoukun, YAN Hui, LIU Yueqin, ZHANG Yingjie. Effects of Different Treatments of Transportation Stress on Energy Metabolism of Lambs Muscle after Slaughter [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5101-5111.
[6]	LI Dengpan, MA Keyan, HAN Jintao, BAI Yaqin, LI Taotao, MA Youji. Detection and Analysis of Whole Genome Selection Signal of Yongdeng Qishan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4577-4588.
[7]	ZHAO Xueyang, LI Danni, WANG Yuchen, GUO Lei, WANG Li, HUANG Jie, JIAO Yiqiang, AN Xiaopeng, ZHANG Xiyun, ZHANG Lei, SONG Yuxuan. GWAS Analysis of Lambing Traits in East Friesian and Hu Crossbred Sheep and Verification of Candidate Gene GRID2 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4625-4635.
[8]	ZHANG Hanyue, ZHAO Dan, LIANG Yu, ZHAO Bishi, FAN Mengdan, QIAO Liying, LIU Jianhua, YANG Kaijie, PAN Yangyang, LIU Wenzhong. miR-150 Regulates Ovine Preadipocyte Differentiation by Targeting AOC3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3262-3274.
[9]	LIU Linli, PENG Xuelan, LI Bo, CHENG Lefan, CIREN Lamu, ZHANG Enping. Effect of Overexpression of UCP3 Gene on the Differentiation of Sahu Sheep Preadipocytes [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3275-3285.
[10]	HE Mingyang, MA Yujing, WANG Yong, YANG Ruochen, LIU Yueqin, ZHANG Yingjie, DUAN Chunhui. Effects of Melatonin on Proliferation, Apoptosis of Ovarian Granulosa Cells, and Its Secretion of Steroid Hormones of Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3313-3324.
[11]	SONG Meijun, HAO Kexing, HAI Siyu, CHEN Yan, WANG Jing, HU Guangdong. Effects of SRIF-14 on Proliferation and Apoptosis of Endometrial Epithelial Cells in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3325-3334.
[12]	LI Yuexin, LIU Aiju, MA Xiaofei, ZHENG Zhong, HU Boxin, ZHI Yunxia, TIAN Shujun. Effect of TGFβR1 on the Function of Sheep Granulosa Cells Mediated by TGF-β/Smad Signaling Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3335-3347.
[13]	MA Youji, CHEN Pengfei, MA Qing, WU Yi. Effects of Different Exercise Amounts on Rumen Microflora Diversity of Tan Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3393-3405.
[14]	WANG Wannian, CHEN Sijia, GAO Jinrong, WEN Zhonghao, YUAN Mengjiao, ZHANG Hongzhi, PANG Zhixu, QIAO Liying, LIU Wenzhong. Simulation Study on Genomic Selection of Sex-limited Traits Using Multilayer Perceptron in Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2824-2835.
[15]	XIONG Chengkun, ZHANG Daoliang, YANG Yue, DING Hongyan, ZHAO Jie, LI Yu, WANG Xichun, FENG Shibin, ZHAO Chang, TANG Jishun, WU Jinjie. Effect of Rutin on Rumen Fermentation, Rumen Flora Structure and Antioxidant Properties in Perinatal Hu Sheep [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2898-2909.

Effects of Diet Energy and Protein Levels on the mRNA Expression of PPARγ and FABP4 in Adipose Tissues of Tan Sheep

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments